You will have to stimulate kittens 2 weeks and under to use the restroom. Kittens older than 2 weeks will be able to urinate and defecate on their own. Their mother would normally lick the genital area; you can mimic this by using a warm moist wash cloth or cotton ball. Do this before and after every feeding. Don’t worry if the kitten does not defecate right away it could take a couple feedings. Remember to dry the kittens after so they will not become chilled. If you notice a kitten trying to nurse on another kitten they will need to be separate immediately this can cause bruising, skin infections, and urinary tract infections.The color of the urine should be a pale amber color if it is dark yellow this could be a sign that the kitten is dehydrated. Kittens older than 2 weeks will be able to urinate and defecate on their own.